Review



mouse embryonic fibroblast mef cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    ATCC mouse embryonic fibroblast mef cells
    Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mef+cells/MEF/pm41811437-212-20-25
    Average 95 stars, based on 563 article reviews
    mouse embryonic fibroblast mef cells - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction
    Article Snippet: .. Mouse Embryonic Fibroblasts (MEF) cells and Human HT1080 cells (ATCC) were cultured in DMEM (Corning) complete with 10% fetal bovine serum (FBS; HyClone) and 1% penicillin/streptomycin (Corning). ..

    Article Title: Identification of an N-terminal Truncation of the NF-?B p65 Subunit That Specifically Modulates Ribosomal Protein S3-dependent NF-?B Gene Expression
    Article Snippet: .. Jurkat A3, HEK293T, and mouse embryonic fibroblasts (MEF) cells (ATCC) were cultured in RPMI 1640 and DMEM, respectively, supplemented with 10% fetal calf serum, 2 m m glutamine, and 100 units/ml each of penicillin and streptomycin. .. IκBα (C-21, sc-371), C-terminal p65 (C-20, sc-372), N-terminal p65 (F-6, sc-8008x), and Sam68 (Src-associated in mitosis 68 kDa; C-20, sc-333) antibodies were from Santa Cruz Biotechnology; GFP (7.1 and 13.1, 11814460001) antibody was from Roche Applied Science; RNA polymerase II (CTD4H8, 05-623) antibody was from Millipore; β-actin (AC-15, A5441) and importin-α (IM-75, I1784) antibodies were from Sigma; poly(ADP-ribose) polymerase (C2–10, 556362), Hsp90 (68, 610418), caspase 3 (19, 610322), CD3 (HIT3a, 550367), and CD28 (CD28.2, 555726) antibodies were from BD; phospho-IκBα (5A5, 9246) and cleaved caspase 3 (Asp-175, 9661) antibodies were from Cell Signaling Technology; RPS3 and Ser-209 phosphorylated RPS3 antibodies were generated and affinity-purified by Primm Biotech, as previously described ( 16 , 17 ).

    Article Title: Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction
    Article Snippet: .. Mouse Embryonic Fibroblasts (MEF) cells and Human HT1080 cells (ATCC) were cultured in DMEM (Corning) complete with 10% fetal bovine serum (FBS; HyClone) and 1% penicillin/streptomycin (Corning). ..

    Control:

    Article Title: Rap1-GTPases control mTORC1 activity by coordinating lysosome organization with amino acid availability
    Article Snippet: .. HELA, HEK293T, U2OS, mouse embryonic fibroblasts (MEF) cells deficient in GCN2 and their littermate-derived wild-type control cells were obtained from ATCC. .. HEK293A cells were purchased from Thermo Fisher Scientific.

    Article Title: Rap1-GTPases control mTORC1 activity by coordinating lysosome organization with amino acid availability.
    Article Snippet: .. HELA, HEK293T, U2OS, mouse embryonic fibroblasts (MEF) cells deficient in GCN2 and their littermate-derived wild-type control cells were obtained from ATCC. .. HEK293A cells were purchased from Thermo Fisher Scientific.

    Mutagenesis:

    Article Title: Base Excision Repair in Mitotic Cells and the Role of Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Post-Mitotic Transcriptional Reactivation of Genes
    Article Snippet: For nascent RNA detection in the cells, a Click-iT TM RNA Alexa Fluor 594 Imaging kit (ThermoFisher Scientific; Cat # C10330) was used. .. The human mutant KRAS expressing pancreatic ductal adenocarcinoma MIA PaCa-2 (ATCC, Manassas, VA, USA, Cat # CRM-CRL-1420) cell line, mouse embryonic fibroblasts (MEF) cells, and BJ-5ta (BJ-hTERT; ATCC, Manassas, VA, USA, Cat # CRL-4001) cells were all maintained in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM; ThermoFisher Scientific, Cat # 11965084) supplemented with 10% fetal bovine serum (FBS; Sigma, Catalog # F2442) and an antibiotic mixture of 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco-BRL, Grand Island, NY, USA). .. The human colon cancer cell line HCT116 (ATCC, Cat # CCL-247) was maintained in McCoy’s 5A (modified) medium (Gibco; Grand Island, NY, USA, Cat # 16600082) supplemented with 10% fetal bovine serum and an antibiotic mixture of 100 U/mL penicillin and 100 μg/mL streptomycin.

    Expressing:

    Article Title: Base Excision Repair in Mitotic Cells and the Role of Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Post-Mitotic Transcriptional Reactivation of Genes
    Article Snippet: For nascent RNA detection in the cells, a Click-iT TM RNA Alexa Fluor 594 Imaging kit (ThermoFisher Scientific; Cat # C10330) was used. .. The human mutant KRAS expressing pancreatic ductal adenocarcinoma MIA PaCa-2 (ATCC, Manassas, VA, USA, Cat # CRM-CRL-1420) cell line, mouse embryonic fibroblasts (MEF) cells, and BJ-5ta (BJ-hTERT; ATCC, Manassas, VA, USA, Cat # CRL-4001) cells were all maintained in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM; ThermoFisher Scientific, Cat # 11965084) supplemented with 10% fetal bovine serum (FBS; Sigma, Catalog # F2442) and an antibiotic mixture of 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco-BRL, Grand Island, NY, USA). .. The human colon cancer cell line HCT116 (ATCC, Cat # CCL-247) was maintained in McCoy’s 5A (modified) medium (Gibco; Grand Island, NY, USA, Cat # 16600082) supplemented with 10% fetal bovine serum and an antibiotic mixture of 100 U/mL penicillin and 100 μg/mL streptomycin.

    Modification:

    Article Title: Base Excision Repair in Mitotic Cells and the Role of Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Post-Mitotic Transcriptional Reactivation of Genes
    Article Snippet: For nascent RNA detection in the cells, a Click-iT TM RNA Alexa Fluor 594 Imaging kit (ThermoFisher Scientific; Cat # C10330) was used. .. The human mutant KRAS expressing pancreatic ductal adenocarcinoma MIA PaCa-2 (ATCC, Manassas, VA, USA, Cat # CRM-CRL-1420) cell line, mouse embryonic fibroblasts (MEF) cells, and BJ-5ta (BJ-hTERT; ATCC, Manassas, VA, USA, Cat # CRL-4001) cells were all maintained in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM; ThermoFisher Scientific, Cat # 11965084) supplemented with 10% fetal bovine serum (FBS; Sigma, Catalog # F2442) and an antibiotic mixture of 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco-BRL, Grand Island, NY, USA). .. The human colon cancer cell line HCT116 (ATCC, Cat # CCL-247) was maintained in McCoy’s 5A (modified) medium (Gibco; Grand Island, NY, USA, Cat # 16600082) supplemented with 10% fetal bovine serum and an antibiotic mixture of 100 U/mL penicillin and 100 μg/mL streptomycin.



    Similar Products

    mouse embryonic fibroblast mef cells  (ATCC)
    95
    ATCC mouse embryonic fibroblast mef cells
    Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mef+cells/MEF/pm41811437-212-20-25
    Average 95 stars, based on 1 article reviews
    mouse embryonic fibroblast mef cells - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    mouse embryonic fibroblasts  (AMS Biotechnology)
    96
    AMS Biotechnology mouse embryonic fibroblasts
    Mouse Embryonic Fibroblasts, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mef+cells/C57BL%2F6+Mouse+Embryonic+Fibroblasts+(MEF)+feeder+cells%2C+irradiated/pm41794018-46-24-27
    Average 96 stars, based on 1 article reviews
    mouse embryonic fibroblasts - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    mouse embryonic fibroblast mef cell line  (ATCC)
    95
    ATCC mouse embryonic fibroblast mef cell line
    Mouse Embryonic Fibroblast Mef Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mef+cells/MEF-1%3B+Embryonic+Fibroblast%3B+Mouse/pmc12959275-38-1-11
    Average 95 stars, based on 1 article reviews
    mouse embryonic fibroblast mef cell line - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    mouse embryonic fibroblast cells  (ATCC)
    99
    ATCC mouse embryonic fibroblast cells
    (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
    Mouse Embryonic Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mef+cells/Wt+MEFs/pmc12818678-207-0-5
    Average 99 stars, based on 1 article reviews
    mouse embryonic fibroblast cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    mef cells mouse embryonic fibroblasts cf 1  (ATCC)
    95
    ATCC mef cells mouse embryonic fibroblasts cf 1
    (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
    Mef Cells Mouse Embryonic Fibroblasts Cf 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mef+cells/MEF-1%3B+Embryonic+Fibroblast%3B+Mouse/10__1016_slash_j__jddst__2026__107992-127-0-7
    Average 95 stars, based on 1 article reviews
    mef cells mouse embryonic fibroblasts cf 1 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Journal: PLOS Pathogens

    Article Title: Nucleus softens during herpesvirus infection

    doi: 10.1371/journal.ppat.1013873

    Figure Lengend Snippet: (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Article Snippet: Mouse embryonic fibroblast cells (MEF, ATCC CRL-2991) and African green monkey kidney cells (Vero, ATCC CCL-81) were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, L-glutamine, and penicillin-streptomycin (Gibco-Invitrogen) at 37 °C in the presence of 5% CO 2.

    Techniques: Tomography, Infection, Electron Microscopy, Standard Deviation, Labeling